RNA sequences amplified from a HBV-genome-encoding plasmid

We collected sequences amplified from three HBV-genome-encoding plasmids, one wild type and two with predefined point mutations. The data was generated to validate a variant-calling strategy of HBV genomes based on RNA-sequencing data. We cloned part of HBV genome into a commercially available plasmid. We introduced predefined point mutations for benchmarking purposes, and validated them. Wild-type- and mutant-containing plasmids were mixed in pre-defined proportions and amplified to generate HBV transcripts. We applied RNA-sequencing, generated reads, and used the software *HBVouroboros* that we developed to perform de-novo assembly of the genome and variant calling. The results demonstrate that the RNA-sequencing + HBVourboros approach recalls HBV genomic variants with high fidelity.