Chip-seq of H3K4me3, H3K4me1, H3K27ac and P300 in HCC cells

Considered as fundamental epigenetic regulators controlling many key cellular processes, histone modifications are a well-conserved and widely studied class of epigenetic modifications. Genome-wide studies have identified enhancers as DNA sequences that bind to H3K4me1 and H3K27ac and promoters as DNA sequences that bind to H3K4me3. To explore how the Twist1 complex (Twist1/YY1/p300) regulates miR-9 expression, we performed ChIP-seq in PLC-PRF-5 cells, providing a panorama of p300, H3K4me3, H3K4me1, and H3K27ac. Overall design: ChIP-seq of p300, H3K4me3, H3K4me1, and H3K27ac was performed in PLC-PRF-5 cells.