Dynamic regulation of miRNA accumulation during C. elegans larval development [smallRNA-Seq 4]. Dynamic regulation of miRNA accumulation during C. elegans larval development [smallRNA-Seq 4]

Here, we profile miRNA expression at high temporal resolution (1 hr interval) during C. elegans postembryonic development, which identifies several miRNAs with highly dynamic concentration changes Overall design: Synchronized L1's of N2 and HW2643 (mir-235(xe181[seed_mutant]) were plated on 10 cm plates (2000 worms/plate) and kept at 25°C for the time-course. Every hour samples were collected from 18h to 30h, extracted RNA, treated with DNAse and subjected to small-RNA sequencing