Oryza sativa cultivar:Oryza sativa japonica Transcriptome or Gene expression

To exploit the advantage of PacBio sequencing's long reads as possible in full-length transcripts study, we used Hiseq2000 paired-end reads to correct CCSs with different minimum predicated accuracy (MPA) rather than minimum of 2 passes of the inserts and validated the full-length splicing isoforms by verifying the chained splicing junctions with at least 2 uniquely mapped Hiseq2000 reads. Our study provides a valuable alternative approach to efficiently identify and validate full-length splicing isoforms on a massive scale in higher eukaryotes with reference genome.