Mass spectrometry on tRNAs from Tet1/2/3 tKO mESCs rescued with TET2 WT or catalytic mutant (HxD) and control (GFP)

Mass spectrometry analysis on tRNAs gel purified from Tet1/2/3 triple KO mouse embryonic stem cells after transient transfection of TET2 catalytic fragment wild type (WT), catalytic mutant (HxD), or a GFP as a negative control. As shown in Fig. 4D of "<i>TET2 chemically modifies tRNAs and regulates tRNA fragment levels</i>" by He et al.

本数据集针对从经瞬时转染TET2催化片段野生型（wild type, WT）、催化突变体（catalytic mutant, HxD）或以绿色荧光蛋白（green fluorescent protein, GFP）作为阴性对照的Tet1/2/3三重敲除（triple knockout, KO）小鼠胚胎干细胞中凝胶纯化得到的转运RNA（transfer RNA, tRNA）开展质谱分析。该数据集对应He等人发表的论文《TET2对转运RNA进行化学修饰并调控tRNA片段水平（TET2 chemically modifies tRNAs and regulates tRNA fragment levels）》中的图4D。