Nfkbid-overexpression and -ablation enhances thymic deletion of autoreactive AI4 CD8 T-cells

Purpose: Analyze genes involved in the negative selection of CD4CD8 double positive thymocytes from NOD-AI4 control mice, NOD-AI4-Nfkbid-/- (KO) and NOD-AI4-60A undergoing negative selection. Methods: RNA-Seq based gene expression analyses were used to identify differentially expressed genes in CD4CD8 double positive thymocytes from NOD-AI4 (control), NOD-AI4-Nfkbid-/- (KO) and NOD-AI4-60A undergoing negative selection. mRNA profiles of CD4CD8 double positive thymocytes from 6-7-week-old NOD-AI4 (control, CTL), NOD-AI4-Nfkbid-/- (knockout, KO) and NOD-AI4-60A (Nfkbid transgenic) mice