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library construction protocol for ultralow RNA-seq method

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NIAID Data Ecosystem2026-03-12 收录
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We present a highly sensitive library construction protocol suitable for subcellular or ultralow input RNA sequencing (ulRNA-seq). In order to improve the sensitivity and low abundance gene detection ability of the library construction protocol, the key components of the library construction protocol, such as reverse transcriptase (RT), template-switching oligos (TSO) terminal modification and template RNA structure were optimized.

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2021-06-27
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