Denovo assembly of a Japan Sea stickleback (Gasterosteus nipponicus) and a Japanese Pacific Ocean lineage of three-spined stickleback (Gasterosteus aculeatus)

DNA within the nucleus is organized into a well-regulated three-dimensional (3D) structure. However, it remains largely elusive how such 3D genome structures influence speciation processes. Recent studies have shown that 3D genome structures influence mutation rates, including the occurrence of chromosomal rearrangement. For example, breakpoints of chromosomal rearrangements are often enriched at topologically associating domain (TAD) boundaries. Here, we hypothesized that TAD structures may constrain the location of chromosomal inversions and thereby shape the genomic landscape of divergence between species with ongoing gene flow, because inversions can contribute to barriers to gene flow. To test this hypothesis, we used a Japanese stickleback species pair, Gasterosteus nipponicus (Japan Sea stickleback) and G. aculeatus (three-spined stickleback). We first constructed high-quality genome assemblies of both species using PacBio HiFi and Dovetail Omni-C technologies and ..., , , # Genome assemblies of Gasterosteus nipponicus and Gasterosteus aculeatus used in \"3D genome constrains breakpoints of inversions that can act as barriers to gene flow in the stickleback\"

We assembled genomes of the two Gasterosteus species in Japan; G. nipponicus and G. aculeatus. We used PacBio HiFi and Dovetail Omni-C technologies to make chromosome-scale assemblies.

## General information

[Access this dataset on Dryad](https://doi.org/10.5061/dryad.ncjsxkt65)

1. Title of Dataset: Genome assemblies of Gasterosteus nipponicus and Gasterosteus aculeatus
2. Author Information

* Principal Investigator
Name: Jun Kitano
Institution: National Institute of Genetics
Address: Mishima, Shizuoka, Japan
Email: [[e-mail]](mailto:[e-mail])
* Data curation
Name: Yo Yamasaki
Institution: National Institute of Genetics
Address: Mishima, Shizuoka, Japan
Email: [[e-mail]](mailto:[e-mail])

1. Date of sample collection

* G. nipponicus: 15/5/2023...,