RNA-seq analysis of wild type and ?bysR in Burkholderia sp. JP2-270

Purpose: In order to identify the differentially expressed genes between wild type and ?bysR, we performed RNA-seq analysis.Methods:All the isolates were cultured in CM medium for 24 h at 28?. Three repetitions were performed for each treatment. The samples were sequenced on an Illumina Hiseq 2000 platform.Clean reads were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. Results:A total of 9-13 million clean reads were obtained per replicate, each read with an average length of 150 bp. The square (R2) of Pearson correlation coefficient is greater than 0.903 between samples (supplementary Fig. S1) indicated that the data had a good reproducibility. Differential expression analysis of samples from WT and ?bysR was performed, and revealed 350 differentially expressed genes (DEGs) consisting of up-regulated (302) and down-regulated genes (48) (with p-adj<0.05, |log2(FoldChange)|> 1) in ?bysR compared to the WT. Overall design: Total mRNA profiles of 24 h old wild type (WT) and ?bysR of Burkholderia sp. JP2-270