Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus

We report RNA sequencing of single Agrp neurons isolated from the arcuate nucleus of the hypothalamus. Analyses of Agrp neuron transcriptomes reveals differential expression of receptors for multiple neuromodulators. Neuroanatomical mapping of known ligands of the receptors define subsets of neurons directly upstream of AgRP neurons in specific brain areas. Isolation of fluorescently labelled individuals cells dissociated from the mouse arcuate nuclues. Cells were visualized and isolated using a micropipette under a microscope, followed by cell lysis, RT, and cDNA synthesis.