Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus

We report RNA sequencing of single Agrp neurons isolated from the arcuate nucleus of the hypothalamus. Analyses of Agrp neuron transcriptomes reveals differential expression of receptors for multiple neuromodulators. Neuroanatomical mapping of known ligands of the receptors define subsets of neurons directly upstream of AgRP neurons in specific brain areas. Isolation of fluorescently labelled individuals cells dissociated from the mouse arcuate nuclues. Cells were visualized and isolated using a micropipette under a microscope, followed by cell lysis, RT, and cDNA synthesis.本研究报道了从下丘脑弓状核（arcuate nucleus of the hypothalamus）分离的单个AgRP神经元（Agrp neurons）的RNA测序数据。对AgRP神经元转录组的分析揭示了多种神经调节因子受体的差异表达情况。通过对该类受体已知配体的神经解剖定位，明确了特定脑区内直接投射至AgRP神经元上游的神经元亚群。本研究涉及从小鼠弓状核解离得到的荧光标记单个细胞的分离流程：细胞通过显微镜下的显微吸管完成可视化与分离，随后依次进行细胞裂解、逆转录（RT）及cDNA合成。