RNA-sequencing for leaves of three garlic varieties

To explore the clove enlarging growth-related genes, we compared the transcriptome of bulbs whose cloves are before enlargement and under enlarging-growth, respectively, using three garlic accessions, Chalingzipisuan, Ershuizao, and Yuanjiangyangsuan. Consequently, 4,658 genes were identified with a differential expression in at least two of three examined accessions. For each accession, bulbs of five plants were pooled as a sample. In addition, three varieties, Chalingzipisuan, Ershuizao, and Yuanjiangyangsuan, were planted in the farm of Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, China, in Oct. of 2019; and in the Feb. and Mar. of 2020, when the bulbs had not initiated to enlarge and were under enlarging growth, respectively, the parts of bulbs were collected. Three individuals were used as three replications