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A Pipeline for High Throughput Concentration Response Modeling of Gene Expression for Toxicogenomics

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE105050
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Concentration Response Modeling on targeted RNAseq (TempOSeq) transcripts from the s1500+ panel utilizing iPSC-Cardiomyocytes treated with four chemicals Fully induced beating cardiomyocytes from the standard donor (Cellular Dynamics) were treated with either Media, DMSO+Media or 3 doses of 4 chemicals. Following 24 hours of incubation, the cell medium was discarded, and cardiomyocytes were lysed with 10μl 1x lysis buffer provided in the TempOSeq assay kit (BioSpyder Technologies, Carlsbad, CA).
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2024-11-25
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