Integrin α5 neutralization in Bone Marrow Macrophages to examine its signaling role in macrophage responses relevant following myocardial infarction

Macrophages infiltrate the infarcted heart and play a critical role in repair, remodeling and fibrosis. Macrophages sense changes in the extracellular matrix (ECM) environment through Integrins, thus activating signaling pathways that regulate their function. Analysis of our previous RNA sequencing data identified integrin α5 (Itgα5) as one of the most upregulated integrin genes in infarct macrophages. Accordingly, we hypothesized that integrin α5 signaling in infarct macrophages transduces ECM-derived signals, regulating responses critical for repair and remodeling of the infarcted heart. In order to study the role of integrin α5 in mmacrophages in vitro, we isolated bone marrow macrophages from WT mice and grow them in fibronectic coated and normal culture dishes, and treated them with a anti-integrin α5 neutralizing antibody and its control (IgG). >>> Submitter declares raw data are unavailable and cannot be recovered. <<<