Data associated with the publication: Dendritic cell activation by iron oxide nanoparticles depends on the extracellular environment

This dataset contains all raw data associated with the study elucidating the mechanisms of nanoparticle-mediated activation of dendritic cells (DCs) when they were exposed to hydroxyethyl starch-coated bionized nanoferrite IONPs (BP) in vitro and in vivo. We observed no evidence of toxicity at doses up to 25 mg Fe/mouse, five-fold higher than those used in subsequent in vivo experiments. Although DCs cultured with BP revealed high levels of nanoparticle uptake, neither JAWSII dendritic cells nor bone marrow derived cells (BMDCs) showed significant changes in marker expression. To assess whether BP interactions in vivo produced different effects, we analyzed CD80, CD86, and MHC II expression of DCs recovered from the livers, spleens, bone marrows, and lymph nodes of mice injected once with BP (5 mg Fe). Interestingly, only DCs in spleens and bone marrows responded to BP exposure. DCs recovered from other tissues and organs showed no evidence of increased activation. These findings highlight complex effects with systemic exposure to nanoparticles, and their potential to mediate specific and selective activation of innate immune cells, which depends on the tissue site and features of the extracellular environment. Our study also emphasize that results obtained from in vitro experiments must be interpreted with caution as they may not faithfully represent responses in living systems. (2024-06)