RNA sequencing of tumor-associated macrophages sorted from OMVs and IN@OMVs-treated mice

To investigate the effects of OMVs and IN@OMVs treatments on mouse tumor-associated macrophages, mouse tumors were harvested after treatment, and CD11b+ F4/80+ labeled cells (TAMs) were sorted by FACS for RNA sequencing. 1×10^6 MC38 cells were injected subcutaneously into the right back of 6–8-week-old female C57BL/6 mice. On day 9, mice were randomly divided into three experimental groups: Control, OMVs (625 μg vesicle protein/kg body weight) and IN@OMVs (625 μg vesicle protein/kg body weight). OMVs, IN@OMVs were injected intratumorally, the control mice were injected intratumorally with an equal volume of solvent, and tumors were harvested from mice after treatment. Next, and the CD11b+ F4/80+ -labeled cells (TAMs) were sorted by FACS. Cellular RNA was isolated from TAMs using the TRIzol reagent and then the samples were immediately shipped frozen for transcriptome sequencing.