Gene expression of S100A10 knocked down human breast cancer cells

The expression level of S100A10 was much higher in the CD44+ cancer cells metastasized to the liver than those at the primary site in the breast cancer patient-derived tumor xenograft mice. Expression level of S100A10 increased by the oncoprotein, PML/RARα and RAS, and its higher expression is linked to worse outcome in a number of cancer types including breast cancers Human breast cancer MDA-MB-231 cells were infected with lentivirus encoding shRNA sequences against S100A10. Two different shRNA sequences (shS100A10-1, shS100A10-5) that effectively knockdown S100A10 were used. Analyses were duplicated.