FIGURE 4 RAW DATA MYD88 MUTANT TRACES

Fluorescence time-traces obtained with disease-associated point mutants of the full-length MyD88 protein, as well as the wild-type full-length MyD88 at a protein concentration of 150 nM. Diffusion time (µs) measured by FCS ADDED ALREADY INTO FIGSHARE FILE FIGURE 2 RAW DATA showing the drastic shift in diffusion time when comparing the mutants to the wild-type protein. (C) Fluorescence intensity histogram showing that the L93P point mutation, which is within the DD, in GFP-tagged MyD88 renders the polymerisation propensity similar to the MyD88 TIRdomain alone. (D) Fluorescence intensity histogram demonstrating that the R196C and L252P point mutations (present within the TIR domain) in the GFP-tagged MyD88 render the polymerisation propensity more similar to the MyD88 DDalone. Fluorescence time-traces and intensity histograms in (A), (C) & (D) are representative of 8 independent experiments. Values in (B) are ±SD from these 8 measurements.