Effect of Chimeric tRNA in Candida albicans genome. Effect of Chimeric tRNA in Candida albicans

we have engineered C. albicans strains that incorporate serine at several non-cognate sites. For this, we have altered the anticodon of a serine tRNA producing chimeric tRNAs similar to the natural tRNACAGSer that incorporates serine at CUG leucine sites. We have produced serine tRNAs with anticodons that read CTA , CTC, CTT (Leucine), ATC (Isoleucine), GCC (Alanine), GGA (Glycine), AAG (Lysine), ACC (Threonine) and TAC (Tyrosine) codons. This strains were evolved by 100 generations and their genome was compared.