HIRA-mediated loading of histone variant H3.3 controls androgen-induced transcription by regulation of AR/BRD4 complex assembly at enhancers [Dex RNA-seq]

To study the function of histone variant H3.3 pathway in prostate cancer (PCa) we knocked out androgen receptor (AR) and H3.3 chaperones HIRA and DAXX in R1-AD1 FLAG-HA-H3F3A cell line. We analyzed the effect of Dexamethasone treatment on the transcriptome in these four cell lines. RNA-seq data for R1-AD1 FLAG-HA-H3F3A (WT androgen receptor) prostate cancer cells and its derivatives (AR KO, HIRA KO, DAXX KO);