Impact of Echs1 on DNA methylome in early mouse embryos

Echs1 plays important roles in histone lysine crotonylation. Although it has been studied in many systems, its functions in early embryos remain unclear. In this study, we knocked down Echs1 in mouse oocytes and examined its impact on epigenome in early mouse embryos including DNA methylome. Generally, siRNA against Echs1 was injected into mouse oocytes, and fertilized oocytes were cultured to early 2-cell stage for DNA methylome analysis by WGBS.