In order to understand the function of Evi5 in iron homeostasis and steroid hormone production, we performed a MALDI-TOF mass spectrometry to identify candidate interacting proteins with Drosophila Ev
Our research showed that PorB can be ubiquitinated in HeLa cells by an unidentified E3 ubiquitination ligase that catalyzes polyubiquitination of PorB. To identify the PorB-interacting E3 ubiquitin li