Whole transcriptomes analyses of gastric tumor tissues derived from Mst4F/F UBCcre (Mst4 KO) mice and wildtype (WT) mice

To explore whether Mst4 limits YAP signaling and suppresses GC development in vivo, RNA sequencing was performed to analyze the genome-wide RNA change in gastric tumors from MST4 KO mice and those from WT mice induced by chemical MNNG. Total mRNA was extracted from gastric tumors of Mst4 KO and WT mice in duplicate. The quality was assessed using Agilent 2100, and the profiles were sequenced on the Illumina Hiseq X10.