Generation of vascularized brain organoids to study neurovascular interactions

In this study, we induced vessel and brain organoids respectively, and then fused two types of organoids together to obtain vascularized brain organoids. We developed an induction approach for brain-specific vascular organoids and performed the single-cell sequencing. The vessel organoids recapitulated the cerebrovascular cell types and cell composition of vessel organoids resembled brain vessels in vivo.