Coagulation parameters of the FIBTEM assay from thromboelastometry analysis of blood samples of different groups of rats.

The animals (n = 6 rats/group) were injected i.d. with Lob (150 µg), Lor (450 µg), or Lca (300 µg), which corresponded to 3 MDD (Minimum Defibrinating Dose) of each extract. One hour (Lob), or two hours (Lor and Lca) later, the animals were treated with LAV or sterile saline (1.5 mL, iv), and blood was collected 24 h after the treatment. Thromboelastography was performed in citrated whole blood with the addition of 0.2 M calcium chloride and specific reagents for the analysis of the FIBTEM. The control group consisted of non-envenomed rats treated with LAV. The data were captured by the ROTEM software in the time interval of 40–60 min. n = 6 rats/ group.