Chlorophyll and phaeopigments measured from discrete bottle samples from CCE LTER process cruises in the California Current System, determined by extraction and bench fluorometry, 2006 - 2017 (ongoing).

Discrete bottle samples taken from various depths in the CCE region are filtered (known volumes) onto GF/F filters onboard the CCE Process cruises (since 2006, ongoing). The filters are placed into culture tubes containing 90% acetone, and the fluorescence of the samples is read on a fluorometer after 24 to 48 hours. The samples are then acidified to degrade the chlorophyll to phaeopigments (non-photosynthetic pigments) and a second reading is taken. The readings prior to and after acidification are used to calculate concentrations of both chlorophyll a and phaeopigments (i.e. phaeophytin).