The kinetic parameters for the synthetic reactions catalyzed by Teth514_1788 and Teth514_1789.

aThe kinetic parameters were calculated by fitting the initial velocities to various concentrations of acceptor substrates in the presence of 10 mm α-Man1P using the Michaelis-Menten equation.b,cThe kinetic parameters were calculated by fitting the initial velocities toward various concentrations of donor substrates in the presence of 10 mm β-1,2-Man2 (b) or 10 mmd-mannose (c) using the Michaelis-Menten equation.dNot detectable. To investigate the acceptor specificities, the synthetic reactions were examined using the following putative carbohydrate acceptors: d-allose, d-altrose, d-arabinose, d-fructose, d-galactosamine, d-galactose, d-galacturonic acid, d-glucosamine, d-glucose, d-glucuronic acid, d-lyxose, d-talose, d-ribose, d-xylose, l-arabinose, l-rhamnose, 1,5-anhydro-d-glucitol, 2-deoxy-d-glucose, methyl-α-d-glucoside, methyl-β-d-glucoside, 3-O-methyl-d-glucose, N-acetyl-d-galactosamine, N-acetyl-d-glucosamine, N-acetyl-d-mannosamine, cellobiose, gentiobiose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose, melibiose, nigerose, N,N′-diacetylchitobiose, sophorose, sucrose, trehalose, and xylobiose.The kinetic parameters for the synthetic reactions catalyzed by Teth514_1788 and Teth514_1789.