TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors. TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors

TET2 and DNMT3A regulate the sensitivity to PARP inhibitor Overall design: To model the somatic loss-of-function mutations found in human blood diseases, mice with conditional inactivation of Dnmt3a and Tet2 in the hematopoietic system were generated and further crossed with mice harboring Flt3ITD mutation. Lin-cKit+ bone marrow cells (BMC) were extracted from bone marrow of mutant and control mice. Extracted BMC were treated with ruxolitinib.