Transcriptome-wide probing of RNA in Bacillus subtilis identified RNA thermometers that regulate translation of two glycerol permease genes

Here we use Structure-seq2 to probe the in vivo RNA structurome of B. subtilis grown at four different temperatures. We show that increases in reactivity with increasing temperature follow non-monotonic trends. Focusing on subregions of the transcriptome led to the discovery of two novel RNA thermometers that control expression of glpF and glpT. Structure-seq2 libraries prepared from RNA extracted from Bacillus subtilis grown to mid-exponential phase at four different temperatures. Control samples and DMS-treated samples were prepared from three biological replicates. Libraries were sequenced on an Illumina NextSeq in single-end mode.