Processed 16S rRNA gut microbiota data: Hizikia fusiforme polysaccharide in OVA-sensitized mice [dataset]

This dataset contains the processed 16S rRNA gut-microbiota data from a study of Hizikia fusiforme polysaccharide (HF) in an ovalbumin (OVA)-induced food-allergy model in female BALB/c mice. Fecal samples were collected from five groups (n = 4 per group; 20 samples total): Control (NC), OVA/OVA (M), OVA/HFP (HF preventive), OVA/HFT (HF therapeutic), and OVA/SM (sunitinib malate positive control). The V4 hypervariable region of the 16S rRNA gene was amplified with primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') and sequenced on an Illumina MiSeq platform (2 × 300 bp, paired-end). Data were processed in QIIME2 (OTU clustering at 0.03 dissimilarity; taxonomy assigned against the reference database). The raw FASTQ reads are no longer available from the sequencing provider; this archive therefore provides the processed data required to reproduce all microbiome analyses, including the OTU/feature tables with taxonomy, relative-abundance tables (phylum to genus), the Bray–Curtis distance matrix, alpha-diversity metrics (Chao1, Shannon, Simpson, Good’s coverage), PCoA coordinates, LEfSe and BugBase outputs, and sample metadata (sample_metadata.csv). See README.txt for file-by-file details. Associated manuscript: “Seaweed-Derived Hizikia fusiforme Polysaccharides Alleviate Th2/Th17-Associated Responses and Reshape the Gut Microbiota in Ovalbumin-Sensitized Mice” (under review).