Confirmation of Affymetrix data by real-time RT-PCR for cellular target genes induced by EBNA-3A.

aUnique gene identification number (ID) according to the National Center for Biotechnology Information (NCBI).
bAs defined by GO-term and/or Refseq (NCBI).
cx-fold induction was calculated from mean expression values of wt and EBNA-3A negative LCLs originating from the Affymetrix gene array hybridization.
dx-fold induction according to real-time RT-PCR data was calculated from mean expression values of 2 independent RNA samples derived from a wt and an EBNA-3A negative LCL, respectively, which were established from donor 3. Note that LCLs from donor 3 were already examined by the Affymetrix screen and represent long-term established B cell lines.
ex-fold induction according to real-time RT-PCR data was calculated from mean expression values of 2 independent RNA samples derived from a wt and an EBNA-3A negative LCL, respectively, which were established from donors 6 and 7. Note that LCLs from donors 6 and 7 represent freshly established LCLs and were analyzed 6 weeks p.i..
fminimum x-fold induction was estimated for genes that were up-regulated from levels below detection limits by setting the value to a minimal transcript level detected for the gene.