Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy (v1.0)

The aim of this work is to study the 3D organization of some population of interneurons within a sample of interest, previously analyzed for physiological information, using two photon fluorescence microscopy (TPFM). We exploit the high axial and radial resolution of TPFM optical sectioning (0.44 x 0.44 x 2 μm³) in combination with a protocol for tissue clearing and labeling to perform the 3D reconstruction of 300 um thick brain sections. We clear the sections with the SWITCH/TDE clearing method and label the samples with three antibodies to co-stain three different populations of inhibitory interneurons: PV (Parvalbumin), SST(Somatostatin), and VIP (Vaso Intestinal Peptide). A new data version of “Molecular characterization of the interneurons in human temporal neocortex by two photon fluorescence microscopy” can be found here: Costantini et al. (2021) [Data set, v1.1] [DOI: 10.25493/ZM3J-6C5](https://doi.org/10.25493%2FZM3J-6C5)