MRPL37 promotes hepatocellular carcinoma progression through modulating mitochondrial energy metabolism

Mitochondrial ribosomal proteins of the large subunit (MRPLs) are critical for mitochondrial protein synthesis and cellular energy metabolism. The role of MRPL37, a member of the MRPL family, in hepatocellular carcinoma (HCC) progression was investigated using SNU-398 HCC cells. In this study, we performed RNA sequencing (RNA-seq) to characterize transcriptomic changes following shRNA-mediated knockdown of MRPL37. The dataset includes RNA profiles from SNU-398 cells with MRPL37 knockdown and corresponding control cells. This analysis aims to elucidate the molecular mechanisms by which MRPL37 regulates mitochondrial function and energy metabolism in HCC. These data provide insights into the role of MRPL37 in HCC progression and may facilitate the identification of novel therapeutic targets for liver cancer. Overall design: SNU-398 hepatocellular carcinoma cells were cultured under standard conditions and subjected to lentiviral transduction with shRNA targeting MRPL37 (shMRPL37) to achieve gene knockdown. A non-targeting shRNA (shControl) was used as a negative control. After selection and validation of knockdown efficiency by qRT-PCR and/or Western blot, total RNA was extracted from both shMRPL37 and shControl cells. Each group included biological replicates (please specify the number of replicates, e.g., n=3 per group). RNA samples with high integrity were used for library preparation and subsequent high-throughput RNA sequencing. The experimental design enables the identification of differentially expressed genes and pathways regulated by MRPL37 in SNU-398 cells, providing insights into the molecular mechanisms underlying its role in mitochondrial function and hepatocellular carcinoma progression.