Gene expression data from UMUC-14 following FGFR3 knock-down using FGFR3 siRNAs

To better understand the molecular mechanisms underlying altered-FGFR3 oncogenic activity in bladder carcinomas, we made use of UMUC-14 cell lines, which endogenously expressed a mutated activated form of FGFR3 (FGFR3-S249C), the growth and transformation of these cell lines being dependent on activated-FGFR3 activity. We conducted a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not with FGFR3 siRNAs. UMUC-14 cells were transfected or not with siRNA FGFR3. mRNA was extracted after 40h and gene expression analysis was assessed using the U133plus2.0 array