De novo assembly of the genome and transcriptome of Trioza erytreae (Hemiptera: Triozidae) and identification of insecticide-related genes. The genome sequence of Trioza erytreae

Trioza erytreae Del Guercio (Hemiptera: Triozidae) is the vector of the bacteria Candidatus Liberibacter africanus, one of the pathogens causing the citrus greening disease known as Huanglonbing. Although insecticide resistance events have not been reported in this pest yet, several studies have detected insecticide-resistant populations of Diaphorina citri, the asian Huanglonbing vector. As the molecular data of this specie is nowadays limited, this study pretends to provide gene and genome sequences through de novo assembly of next-generation sequencing reads. The genome assembly size is 763 Mb, made of 44,802 scaffolds and a scaffold N50 of 28 Kb. Genome annotation, improved by Oxford nanopore technologies (ONT) direct RNA sequencing, retrieved 44,906 protein-coding genes producing 63,313 unique protein products and a total of 147,712 non-coding transcripts. Functional labels could be assigned to 33,985 proteins according to gene ontology (GO). Within these proteins, a total of 389 transcripts have been related to insecticide resistance. By sequence homology with D. citri and Nilaparvata lugens identified genes, 44 transcripts were associated with insecticide resistance. The data obtained here are intended to contribute to future genomic and functional studies that facilitates the identification of this insect and the design of new insecticides and preventive control strategies.