Arabidopsis shoot apex single-cell transcriptomes

Briefly, we harvested the shoot apices from 7-day-old seedlings and 14-day-old plants, and the 3rd leaf (leaf) from 18-day-old plants, respectively. Thetissues were separately digested in the RNase-free enzyme solution for 2 h at room temperature. The protoplasts were filtered 3-4 times with cell strainers, concentrated and washed 3-4 times with 8% mannitol at room temperature. The protoplast viability was determined by trypan blue staining.