Global gene expression profiling in mouse plasma cell tumor precursor and bystander cells revels potential intervention targets for plasma-cell neoplasia. Mus musculus

We extracted nascent plasma cell tumor (PCT) cells from within inflammatory granulomas (OG) isolated from intraperitoneal pristane-injected BALB/c.iMyc Eμ mice at five different time points during tumor progression. We used laser capture micro-dissection to collect incipient PCT cells and analyzed their global gene expression on Affymetrix Mouse Genome 430A microarrays. Two independent studies were performed with different sets of mice Overall design: RNA was extracted from both laser capture samples and whole tissue sections at 5 different times after tumor initiation: 7 days, 17 days, 33 days, 49/46 days and 104/105 days in two independent experiments, studies 1 and 2