Global landscape mapping of chromatin accessibility in therapy-induced senescent human stromal cells

We report the application of an improved assay for transposase-accessible chromatin with high-throughput sequencing in profiling changes of chromatin openning status in senescent human stromal cells. By obtaining over four billion bases of sequence from Tn5 transposase-processed chromosome DNAs, we generated genome-wide chromatin-state maps of human primary prostate stromal cells (with a starting cell number of 50,000). This study provides a framework for the application of ATAC-Seq technique towards spatiotemporal chromatin configuration of human stromal cells upon therapy-induced senescence, which is usually caused by typical DDR in damaged cells. Overall design: Examination of three different cellular states in a primary normal human prosate stromal cell line, including proliferating cells, bleomycin-induced senescent cells and radiation-induced senescent cells.