Revealing biogeographic patterns in genetic diversity of native and invasive plants and their association with soil community diversity in the Chinese coast

Within-species genetic diversity is shaped by multiple evolutionary forces within the confines of geography, and has cascading effects on the biodiversity of other taxa and levels. Invasive species are often initially limited in genetic diversity but still respond rapidly to their new range, possibly through ‘pre-adapted’ genotypes or multiple sources of genetic diversity, but little is known about how their genetic structure differs from that of native species and how it alters the genetic-species diversity relationship. Here, we selected a widespread native species (Phragmites australis) and its co-occurring invasive competitor (Spartina alterniflora) as our model plant species. We investigated the genetic structure of P. australis using two chloroplast fragments and ten nuclear microsatellites in 13 populations along the Chinese coastal wetlands. We discovered a distinct geographical differentiation, showing that the northern and southern populations harbored unique genotypes. We als..., Sampling, genotyping and sequencing of P. australis
We collected 194 individuals of P. australis from 13 sites along the Chinese coast. To avoid collecting the same clone and to get enough genetic variation, we selected five P. australis reed populations within each site, with a distance of at least 1 km between stands. We collected three individuals from each population. All individuals were transplanted with rhizomes in a common garden at the Jiangwan Campus of Fudan University in Shanghai (31.28°N, 121.48°E). After the plants regrew, we collected and dried young leaves from all individuals, and stored them in zip-lock plastic bags with silica gel at room temperature until DNA isolation. We extracted total DNA from the dried leaves according to a modified cetyltrimethylammonium bromide (CTAB) method. We examined the quality and quantity of extracted DNA with 1% agarose gels and a microscope spectrophotometer, and stored DNA at -20℃ until later genotyping and sequencing.
To measure gen..., , # Revealing biogeographic patterns in genetic diversity of native and invasive plants and their association with soil community diversity in the Chinese coast

[https://doi.org/10.5061/dryad.9zw3r22mt](https://doi.org/10.5061/dryad.9zw3r22mt)

**README**
These are descriptions of RawData file in this Dryad upload.
RawData.xlsx contains the following five sheets:
PA\_Haplotype: Haplotype information of *Phragmites australis* individuals

* Column 1: **ID**: Code for the samples.
* Column 2: **Origin**: Site of origin of the samples.
* Column 3: **Latitude**: Latitude (° N) of the site of origin of the samples.
* Column 4: **Longitude**: Longitude (° E) of the site of origin of the samples.
* Column 5: **T**: Locus haplotype (trnT-trnL).
* Column 6: **R**: Locus haplotype (rbcL-psaI).
* Column 7: **Haplotype**: Combined haplotype.

PA\_SSR: SSR genotypes of *Phragmites australis* individuals.

* Column 1: **Sample.Name**: Name for the samples used in our study.
* Column 2: **Marker**: Gen...