Data_Sheet_1_Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets.pdf

Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.

本研究报告指出，三元硫族化合物纳米片对不同长度的寡核苷酸表现出不同的亲和力，并能有效淬灭染料标记的DNA探针的荧光。基于这些发现，作为一种概念验证应用，三元硫族化合物纳米片被用作目标循环扩增生物传感器，展现出在区分单碱基错配方面的高特异性。此简便策略快速且灵敏，适用于单核苷酸多态性的检测。实现了对未标记靶标（250 fM）的超低检测限和高区分比（5%），在完全匹配（突变型）与单碱基错配（野生型）靶标的混合物中。该方法在临床样本中广泛适用于单核苷酸多态性的检测，使其成为基于突变的临床诊断和基因组研究的有前途的工具。