File S1 - The G516T CYP2B6 Germline Polymorphism Affects the Risk of Acute Myeloid Leukemia and Is Associated with Specific Chromosomal Abnormalities

Figures S1 and S2 and Tables S1-S3. G-banded bone marrow karyotypes of AML patients showing a) 46,XX,del(7)(q22q32) b) 46,XX,del(5)(q13q33) c) 47,XY,+8 d) 45,X,-X,t(8;21)(q22;q22). Figure S2. Gel electrophoresis of G516T CYP2B6 genotyping by BsrI PCR-RFLP on a 2% (w/v) agarose gel. The digestion of the 526-bp PCR product of CYP2B6 yields three bands of 241-bp, 268-bp and 17-bp* for the G/G genotype (wild-type) (lanes 1 and 2), two bands of 509-bp and 17-bp for the T/T homozygous mutant genotype (lane 5), and four bands at 509-bp, 241-bp, 268-bp and 17-bp for the heterozygous G/T genotype (lanes 3 and 4). Lane 6: negative BsrI digestion sample (no target DNA). Lane M: DNA ladder N3236S (New England Biolabs, Inc.). *The small restriction fragment of 17bp is not appeared. (DOC)