Supplementary Material for: Analysis of Dopamine receptor D2 Gene Polymorphism and Correlation with Dyslipidemia in the Chinese population

Objective: The study aimed to explore the genotype and allele distributions of dopamine D2-like receptor (DRD2) gene -141C and C957T polymorphisms in the Chinese Han population with dyslipidemia, as well as their association with serum lipid levels. Methods: 150 patients with dyslipidemia and 150 healthy people were recruited as the case and the control group, respectively. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) levels were detected. The target sequence of DRD2 polymorphisms was amplified by polymerase chain reaction (PCR) and genotyped via sanger sequencing. Results: In DRD2 gene C957T (rs6277), three genotypes of CC, CT and TT were detected with the frequencies of 92.67%, 6.67%, 0.67% in dyslipidemia cases, and 83.33%, 14.67%, 2.00% in the controls, respectively. The CT genotype and T allele frequencies were significantly low in the case group relative to the control group. After adjusting to other clinical indicators, the CT genotype of C957T polymorphism (HR = 0.401, 95% CI = 0.181-0.890, P < 0.05) was still related to a significantly reduced risk of dyslipidemia. The C957T CT genotype carriers had the lowest values of serum TC, TG, LDL, and the highest values of serum HDL-C. Conclusion: DRD2 gene C957T polymorphism was an independent influencing factor associated with the susceptibility to dyslipidemia, the CT genotype was associated with decreased odds of susceptibility to dyslipidemia.