Circadian Reprogramming by High-Fat Diet Signifies Temporal Coordination of Metabolic Cycles in the Kidney

The circadian gene expression in peripheral tissue displays rhythmicity which is reprogrammed by feeding rhythms and dietary composition in mammals. In this study, circadian transcriptome was performed to investigate how high fat diet influences circadian gene regulation in the mouse kidney. Overall design: 6-week-old, male C57BL/6 mice were subjected to ad libitum normal chow diet or to high-fat diet under 12hr light/ 12hr dark schedule. Kidney was harvested every 4 hours over the circadian cycle at ZT0, 4, 8, 12, 16, 20 (n=3 per time point per group). Total RNA was extracted from kidney, and used for RNA-seq.