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Suppl. Information to "The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching"

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Zenodo2021-07-21 更新2026-05-25 收录
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<strong>Supplementary File 1:</strong> Multiple alignment for protein sequences of core histones with Pocillopora acuta, Pocillopora damicornis, Acropora digitifera, Nematostella vectensis, Hydra vulgaris, Schistosoma mansoni and Mus musculus. A. Histone H2A; B. Histone H2B; C. Histone H3; D. Histone H4. An asterisk (*) means that the amino acid is conserved between all species. <strong>Supplementary File 2</strong>: Original (uncropped and unedited) images used for Figures 1 to 4. <strong>Supplementary data 3:</strong> <em>P. acuta</em> nuclei and <em>Symbiodinium</em> count on a Thoma cell counting chamber done over three different nuclei extractions. For each extraction, two counts were performed. P. acuta nuclei were stained with Hoechst 33342 and display a blue fluorescence at 350 nm. Symbiodinium are not damaged by our extraction method and are not permeable to Hoechst. They display a red fluorescence because of their chlorophyl content. Observations were done on a Leica DMLB with objective PL Fluotar 40x and 100x. Extraction #1 replicate 1: 102 <em>P. acuta</em> nuclei (Blue) ; 2 <em>Symbiodinium</em> (Red)<br> Extraction #1 replicate 2: 112 <em>P. acuta</em> nuclei (Blue) ; 2 <em>Symbiodinium</em> (Red) Extraction #1 replicate 1: 42 <em>P. acuta </em>nuclei (Blue) ; 0 <em>Symbiodinium</em> (Red)<br> Extraction #1 replicate 2: 55 <em>P. acuta </em>nuclei (Blue) ; 1 <em>Symbiodinium</em> (Red) Extraction #1 replicate 1: 215 <em>P. acuta </em>nuclei (Blue) ; 3 <em>Symbiodinium</em> (Red)<br> Extraction #1 replicate 2: 257 <em>P. acuta</em> nuclei (Blue) ; 5 <em>Symbiodinium</em> (Red) Made at IHPE.

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2021-07-21
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