Source and culture methods of organisms used in the toxicity tests.

1—Minimum essential medium (MEM) containing 10% fetal bovine serum (FBS), 100 mg l−1 penicillin and 100 mg l−1 streptomycin. 2—Dulbecco medium supplemented with 10% heat-inactivated fetal calf serum. 3—Sf-900 II SFM. 4—RPMI-1640, supplemented with 10% heat-inactivated fetal calf serum. 5—Fully aerated seawater at salinity: 33±1‰. 6—Fully aerated 0.22 µm filtered seawater at salinity: 33±1‰. 7—Glass-fiber (GF/C Whatman, Maidstone, UK) filtered freshwater. 8—ISO testing water (1) [36]. 9—Fully aerated 1 µm filtered seawater at salinity: 33±1‰. 10—60 µg ml−1 instant ocean sea salts dissolved in water. 11—f/2 culture medium [37]. 12—Neural basal medium with B27 (Invitrogen, Carlsbad, CA, USA) and 0.5 mM GlutaMAX.