series1_GSE147507 : SARS-CoV-2 (USA-WA1/2020) viral gene expression from infected (MOI 2.0) NHBE Cells

RNA-Seq reads (FASTQ) of biological triplicates from NHBE cells infected with SARS-CoV-2 (USA-WA1/2020, MOI 2.0) (series1_GSE147507) were downloaded from NCBI SRA, and data analysis was performed using CLC Genomics workbench (CLC v20). The reads were first aligned to the human genome (hg38) with annotations (Ensemble v91), and then all unmapped reads were aligned to the SARS-Cov-2 reference genome NC_045512. The expression of each virus gene is quantified and reported as total counts and transcripts per million (TPM).<br>

从NCBI序列读取档案（Sequence Read Archive, SRA）下载了感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2，USA-WA1/2020毒株，感染复数（Multiplicity of Infection, MOI）为2.0）的正常人支气管上皮细胞（Normal Human Bronchial Epithelial, NHBE）三份生物学重复的RNA测序读段（FASTQ格式），对应数据集系列为series1_GSE147507，并采用CLC Genomics Workbench（CLC v20）进行数据分析。首先将读段比对至带有注释信息的人类参考基因组hg38（Ensemble v91），随后将所有未比对上的读段比对至SARS-CoV-2参考基因组NC_045512。对每个病毒基因的表达量进行定量，以总计数（total counts）与每百万转录本数（Transcripts Per Million, TPM）两种形式报告。

搜集汇总

背景与挑战

背景概述

该数据集提供了SARS-CoV-2（USA-WA1/2020株）感染NHBE细胞（MOI 2.0）后的病毒基因表达数据，基于RNA-Seq技术分析。数据来源于NCBI SRA，通过CLC Genomics工作台进行比对和量化，包括人类基因组和SARS-CoV-2参考基因组的对齐，并报告总计数和TPM值。数据集发布于2020年，专注于COVID-19相关研究，适用于生物信息学分析。