HORMONAL CONTRACEPTIVES AND FRUCTOSE DIET REDUCES HIGH DENSITY LIPOPROTEIN CHOLESTEROL IN BLOOD LIPID ESTIMATION IN FEMALE WISTAR RATS

The study evaluated effects of ethinyl estradiol and norgestrel combined hormonal oral
contraceptives doses on blood lipids in fructose fed Female Wistar Rat. Female Rats weighing 94-262g were randomly grouped into four (4) experimental groups (n=6). Prepared 0.15 and 0.3 ml, Duofem combined ethinlyl estradiol and norgestrel oral contraceptives were administered orally (p.o) to fructose fed group A and B animals respectively, while group C animals were fed fructose diet only and the control group D animals were fed normal feed, the procedure lasted for 28 days, twenty four (24) hours after the last treatment, the animals were euthuanized by cervical dislocation, blood samples collected, and lipids profile (total Cholesterol (TCL), triglyceride (TG),High Density Lipoprotein Cholesterol (HDL-C) and Low Density Lipoprotein Cholesterol (LDLC) were estimated. A quantity, 0.3ml ethinlyl estradiol and norgestrel significantly (p<0.05) reduced mean HDL-C level (46.12±2.57 mg/dl) of group A animals compared to control group, on normal diet with mean HDL level of 65.74±3.58mg/dl. Duofem COC produce more severe alteration of lipid homeostasis in Rats on higher combined oral contraceptives (0.3ml) treatment, as a result of insulin-resistance that resulted from chronic fructose consumption.