Transposon sequencing of R. solanacearum infecting tomato plants

<i>Ralstonia solanacearum</i> is an extremely destructive plant pathogenic bacterium without an effective control method. Though many pathogenic factors have been identified, the survival strategies of <i>R. solanacearum</i> in host plants remain misty. To identify <i>R. solanacearum</i> genes required for survival in tomato plants, this study conducted a transposon insertion sequencing (Tn-seq), a high-throughput genetic screening approach, using the in-planta environment as screening stress.