SIRT6 loss causes intervertebral disc degeneration in mice by dysregulating senescence and SASP status

Transcriptional analysis of 24-month-old primary nucleus pulposus (NP) and annulus fibrosus (AF) mouse disc tissues from Sirt6fl/fl and Sirt6cKO mice, which harbor a mutation which constiuitively deletes Sirtuin 6 in aggrecan expressing cells. SIRT6 loss causes intervertebral disc degeneration in mice by dysregulating senescence and SASP status Tissues from the nucleus pulposus (NP) and annulus fibrosus (AF) were harvested from the intervertebral discs of four Sirt6fl/fl and four Sirt6cKO mice at 24 months of age. NP and AF tissues were collected from lumbar and caudal discs in each animal for RNA extraction and hybridization using Affymetrix microarray.