RNA-seq of synchronized S phase or G2 phase cells treated with an ATR inhibitor

We performed RNA-seq in cells synchronized to S or G2 phase to identify genes that were transcriptionally regulated by ATR activity. Overall design: RPE-1 cells were synchronized to late S (4 hours post-release from thymidine block), or to G2 phase (6 hours post-released from thymidine block) and either mock-treated or ATR-inhibited to identify genes that were transcriptionally controlled by ATR. An asynchronous control and an un-released from the thymidine block control were included. These 6 conditions were repeated in 3 independent experiments (total of 18 samples) and analyzed by RNA-seq.