Spn genes tested for their effect on mucus interactions.

Spn surface factors (row 2–5) and enzymes (row 6–10) tested for their effect on mucus interactions. Data shows the results of adherence assays of a Spn gene-knockout strain with mNF or, as noted with hNF compared to wild-type Spn. (-) indicates that there was no increase in adherence and a (+) indicates there was an increase in adherence. We tested these genes for their role in mucus binding in the Type 4 (TIGR4 isolate) background, if an effect was detected, we also tested a knockout mutant in the Type 23F background.